The Gastrointestinal Research Group
Professor, Department of Microbiology, Immunology and Infectious Diseases
Member, Gastrointestinal Research Group
Associate Member, Bacterial Pathogenesis and Mucosal Inflammation Research Groups
Tier 1 Canada Research Chair (CRC) Chair in Mucosal Inflammation
Office: Room 1879, Health Sciences Centre
BSc (Hons), University of Winnipeg
MSc, University of Manitoba
PhD, McGill University
A major effort underway in our laboratory is to understand the mechanisms whereby infectious agents, lipid mediators of inflammation and cytokines modulate innate, immune and mucosal epithelial cell functions. Of particular interest to our group is the emerging concept that cross-talk between pathogen components and host cells or both, can predetermine the outcome of inflammatory or host defense mechanisms. Consistent with this concept, our program of research is divided into three areas as follows: 1. To define the biochemical and molecular interactions by which the mucosal pathogen, E. histolytica trophozoites or its components can modulate epithelial barrier function in the gut. 2. To determine the molecular mechanisms by which PGE2 regulates IL-8 gene expression and protein production in human colonic epithelial cells, and 3. To define the cellular and molecular basis by which E. histolytica adhesin, the Gal-lectin, can stimulate an effective cell-mediated immune response against ameba.
Dharmani P, Leung P, Chadee K. Tumor Necrosis Factor-α and Muc2 Mucin Play Major Roles in Disease Onset and Progression in Dextran Sodium Sulphate-Induced Colitis. PLoS One. 2011;6(9):e25058.
Lejeune M, Moreau F, Chadee K. Prostaglandin E(2) Produced by Entamoeba histolytica Signals via EP4 Receptor and Alters Claudin-4 to Increase Ion Permeability of Tight Junctions. Am J Pathol. 2011 Aug;179(2):807-18
Hou Y, Mortimer L, Chadee K. Entamoeba histolytica cysteine proteinase 5 binds integrin on colonic cells and stimulates NFkappaB-mediated pro-inflammatory responses. J Biol Chem. 285(46):35497-504, 2010
Lejeune M, Leung P, Beck PL, Chadee K. Role of EP4 receptor and prostaglandin transporter in prostaglandin E2-induced alteration in colonic epithelial barrier integrity. Am J Physiol Gastrointest Liver Physiol. 299(5):G1097-105, 2010
Dey I, Giembycz MD, Chadee K. Prostaglandin E(2) couples through EP(4) prostanoid receptors to induce IL-8 production in human colonic epithelial cell lines. Br J Pharmacol 156(3): 475-85, 2009
Ivory CP, Prystajecky M, Jobin C, Chadee K. TLR-9 dependent macrophage activation by Entamoeba histolytica DNA. Infect Immun 76(1):289-97. 2008
Ivory CP, Chadee K. Activation of dendritic cells by the Gal-lectin of Entamoeba histolytica drives Th1 responses in vitro and in vivo. Eur J Immunol 37(2): 385-94, 2007.
Kammanadiminti SJ, Chadee K. Suppression of NF-kappa B activation by entameba histolytica in intestinal epithelial cells is mediated by heat shock protein 27. J Biol Chem. 281(36):26112-20. 2006
Lidell ME, Moncada DM, Chadee K, Hansson GC. Entamoeba histolytica cysteine proteases cleave the MUC2 mucin in its C-terminal domain and dissolve the protective colonic mucus gel. Proc Natl Acad Sci U S A. 103(24):9298-303. 2006
Moncada D, Keller K, Ankri S, Mirelman D, Chadee K. Antisense inhibition of Entamoeba histolytica cysteine proteases inhibits colonic mucus degradation. Gastroenterology. 130(3):721-30. 2006
France Moreau email@example.com