University Core DNA Services
We are a multi-user core facility and provide custom oligonucleotide (DNA and RNA) synthesis and automated DNA sequencing and fragment analysis services to both academic and commercial researchers. These services are not restricted to the University of Calgary and are also available to external academic and commercial researchers.
DNA Synthesis, to 120 bases long
RNA Synthesis, including 2'-O-methyl RNA and 2'-O-fluoro RNA nucleosides, and siRNA (RNAi)
Locked Nucleic Acids (LNA)
Phosphorothioate antisense oligonucleotides
Dye labeled and dual-labeled oligonucleotides
We are specialists in nucleic acid chemistry and solid-phase synthesis and regularly prepare a wide range of unusual or highly modified oligonucleotides that are difficult or very expensive to obtain from other sources.
Applied Biosystems 3730XL 96 capillary sequencer
DNA sequencing from a wide range of templates for small and large-scale projects
Wide range of fragment analyses including SNPs, microsatellites, AFLP, T-RFLP, SSCP, relative fluorescent quantitation, mapping, and fingerprinting
Use our online dnaLIMS system for submitting sequencing requests and retrieving data at http://www.sequencing.ucalgary.ca/
Life Technologies 5500xl genome analyzers (2 ea)
Dual six-lane independent FlowChips on each 5500xl
Whole genome and whole exome sequencing
Whole transcriptome sequencing (RNA-Seq)
Small RNA analysis
Massively parallel (next-generation) DNA sequencing services are offered through the ACHRI Genomics and Bioinformatics Platform which is co-located within the same sequencing laboratory (HSC B104A), but administered by the Alberta Children's Hospital Research Institute (ACHRI) in the Faculty of Medicine. The genomics facility is co-funded by both ACHRI and the Faculty of Medicine and next-generation DNA sequencing services are available to all members of the University of Calgary community for the same service fees.
UCDNA Services, Faculty of Medicine, University of Calgary
3350 Hospital Drive NW, Calgary, AB, Canada T2N 4N1
Synthesis Lab: tel. 403-220-4277; e-mail dnalab [at] ucalgary [dot] ca
Sequencing Lab: tel. 403-220-4503; e-mail dnaseq [at] ucalgary [dot] ca