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David Proud

Submitted by wcm on Fri, 08/29/2008 - 9:25pm

Basic Information







Last Name: 

Contact Information

Office: HRIC 4AC60 (403) 210-3816
Laboratory: HRIC 4C50-54 (403) 210-9479

(403) 270-0692


Research Interests: 

Human rhinoviruses are major trigger factors for acute exacerbations of asthma and chronic obstructive pulmonary disease (COPD). It is unclear how rhinovirus infections induce disease exacerbations, but the airway epithelial cell is the principal site of rhinovirus infection. Rhinovirus infection does not cause overt epithelial cytotoxicity, so current thinking is that rhinoviruses induced disease by altering epithelial cell biology in a manner that increases airway inflammation. We are using both in vitrostudies with cultured epithelial cells, as well as natural rhinovirus infections in humans to examine the mechanisms by which infected cells produce both host defense molecules, which may protect against viral infections and inflammation, and proinflammatory cytokines that could worsen inflammation. We are trying to determine if the balance between these two responses is critical in determining susceptibility to asthma attacks. Newer studies are extending this work to examine the role of rhinovirus in causing structural changes in the airway, and to examine how cigarette smoking may alter epithelial responses to rhinovirus infections.

Key words describing research:

airway inflammation, asthma, COPD, rhinovirus, epithelial cell biology, chemokines, antivirals, host defense, growth factors

Research Funding: 
Canada Research Chair (CRC)
Canadian Institutes of Health Research (CIHR)
US National Institutes of Health
AstraZeneca AB
Techniques used in your laboratory: 

cell culture, viral purification, viral titer assays, ELISA, real-time RT-PCR, western blotting, promoter-luciferase constructs, electrophoretic mobility shift assays, siRNA knockdown, in vivo studies.


List of laboratory personnel: 
Shahina Wiehler, Research Associate
Suzanne Traves, Research Associate
Magda Hudy, Graduate student
Christopher Shelfoon (Graduate Student) email:
Danielle Minor (Graduate student) email:
Barbara Maciejewski (Graduate Student) email:
Sami Shariff (Graduate Student) email:
Sergei Nikitenko (Graduate Student) email:
Jason Warnason (Graduate Student) email:
Administrative assistant: 

Angela Nell, (403) 220-8857